05/05/2020
Multiple Comparisons
| Legend | Name | Description |
|---|---|---|
| A | New group | Adds a comparison group. The name must be unique. |
| B | Groups | List of added comparison groups. |
| C | Comparison modes | Defines the possible combinations of comparison between each group. See more information in the Remarks section. |
| D | Experiment group | The experimental sample group from the selected comparison group. |
| E | Available samples | Available samples to be distributed to the sample groups. In this comparison type, samples can be included in multiple sample groups. |
| F | Control group | The control sample group from the selected comparison group. If the "Control First" option is checked, this sample group will always represent the control group from the first comparison group. |
| G | Arrows from/to experimental group | Distributes the samples to or from the experimental group. Single arrows will transfer the selected samples. Double arrows will transfer all samples to the target group. |
| H | Arrows from/to control group | Distributes the samples to or from the control group. Single arrows will transfer the selected samples. Double arrows will transfer all samples to the target group. |
| I | Sample information | Brief information about a selected sample, including its title, identifier and Series accession ID, if available. |
| J | Probe annotations | List of annotation columns that can be included in the final results table. Only available if the platform data was loaded in the current Session. |
| K | Advanced options | Options for statistical correction of the final results. |
| L | Start analysis | Starts the DE analysis using the selected samples and options. |
Right-click menu in sample groups
| Legend | Name | Description |
|---|---|---|
| A | View as | Displays the sample names according to the GEO metadata entries, if present. |
| B | Auto-distribute dual-channel samples | When working with dual-channel datasets, each item in the list will represent a sample channel (i.e., half sample) instead of an integral sample. This option ensures that a sample channel distributed to a group will cause the other channel to be moved to the opposite group. This way, both channels will be present in both groups. |
Remarks
The Multiple Comparison type is used to compare pairs of sample groups, each one between an experimental group and a control group. In other words, one comparison group is defined by two sample groups being compared.
In addition, there are comparison modes that include more comparison combinations to each comparison group.
The comparison modes are described below:
- Per group: For each comparison group, the samples are compared between the Experimental and Control group.
- First control: For each comparison group, the samples are compared between the Experimental and the first Control group.
- Crossed: In addition to the comparisons "Per group", the Experiment from each comparison group will be compared to the Control group from the other comparison groups.
- Lateral: In addition to the comparisons "Per group", the Experiment of each comparison group will be compared to the Experiment from the other comparison groups, and the Control between the other Control groups.
- Combinatorial: Combines the "Per Group", "Crossed" and "Laterial" comparison modes.